Cloning and Characterization of the cDNA Encoding the α-Subunit of cGMP- Phosphodiesterase in Canine Retinal Rod Photoreceptor Cells

Cloning and Characterization of the cDNA Encoding the α-Subunit of cGMP-Phosphodiesterase in Canine Retinal Rod Photoreceptor Cells

Cloning and characterization of the cDNA encoding the alpha-subunit of cGMP-phosphodiesterase in canine retinal rod photoreceptor cells.

Cloning and Characterization of the cDNA Encoding the α-Sub-

Correspondence to: Kunal Ray, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853; email: [email protected] Rod photoreceptor cyclic GMP-phosphodiesterase (cGMP-PDE, EC 3.1.4.17) is a key enzyme in the phototransduction cascade of the vertebrate retina. The enzyme is composed of αand beta-catalytic subunits, and two identical inhibitory gamma-subunits. Once the phototransductio...

Bovine cone photoreceptor cGMP phosphodiesterase structure deduced from a cDNA clone.

A full-length cDNA clone encoding the alpha' subunit of cGMP phosphodiesterase (PDE) from bovine cone photoreceptors was selected by probing a retinal library with a DNA fragment encoding the catalytic core of the rod cGMP PDE alpha subunit. Identity of the clone was confirmed by comparing its deduced sequence with cone PDE peptide sequences determined by Charbonneau et al. [Charbonneau, H., Pr...

Irish setter dogs affected with rod/cone dysplasia contain a nonsense mutation in the rod cGMP phosphodiesterase beta-subunit gene.

Irish setter dogs affected with a rod/cone dysplasia (locus designation, rcd1) display markedly elevated levels of retinal cGMP during postnatal development. The photoreceptor degeneration commences approximately 25 days after birth and culminates at about 1 year when the population of rods and cones is depleted. A histone-sensitive retinal cGMP phosphodiesterase (PDE; EC 3.1.4.35) activity, a ...